AFP and hepatocellular carcinoma: After 24 hours of wounding, AFP- HCC cells (Mahlavu, SNU449, SNU475, SNU182) moved through the wound, whereas AFP+ HCC cells (Huh7, Hep3B, Hep G2, Hep40) cells did not (Figure 3b; Hep40 and SNU182 data not shown).