Among Wnt ligands and Frizzled receptors that we found to be expressed or upregulated in HCC cell lines, Wnt3, Wnt4, Wnt5a, Fzd3, Fzd6 and Fzd7 have been previously reported to be overexpressed also in primary HCC tumors [55-57]. The gene discussed is WNT4; the disease is hepatocellular carcinoma.