39 Mouse muscle tissue was the third highest tissue in terms of CAT reporter gene expression when mice were injected intravenously with DOTAP:Chol complexed with the CAT reporter plasmid.31 This formulation of liposomes has been shown to penetrate several tight layers of smooth muscle cells in the arteries of pigs40 and has been used for gene delivery of various tumor suppressor genes with evidence of effectiveness in treatment of primary and disseminated lung cancers.41,42. This evidence concerns the gene CAT and lung cancer.