Cross-reactivity with adenovirus, CMV, EBV, echinococcus, HBV, influenza A-B, mycoplasma pneumoniae, picorna, syphilis, rubella, toxoplasma and varicella zoster virus was negative (these technical characteristics refer to the kit that is used in our hospital (Novatec (Dietzenbach, Germany) HSV type 1 and type 2 IgG, IgM-ELISA) [15]. This evidence concerns the gene CD40LG and rubella.