We further transiently overexpressed angiogenic genes VEGF and PDGF in ex vivo expanded stem cells using a bicistronic vector, in which both VEGF-A164 and PDGF-BB genes are within the internal ribosomal entry site under CMV promoter, to augment stem cell effect for treatment of myocardial ischemia induced in rat model. The gene discussed is VEGFA; the disease is myocardial ischemia.