When the Sha 31 monoclonal antibody was used for PrPres detection, the usual 3-band pattern (between 18–20 and 30 kDa), that became a single band pattern (18–20 kDa) after PNGase deglycosylation, was observed in all TgOvPrP4 and C57Bl/6 PrPres positive mice inoculated with classical scrapie or BSE (groups 2, 3, 4 and 5) (data not shown). Here, NGLY1 is linked to scrapie.