Using immunocytochemistry on formalin fixed paraffin embedded GC cell lines, p65 protein expression was more frequently observed in NF-κB/on GC cell lines compared to NF-κB/off GC cell lines in terms of nuclear sublocalization, percentages of cells with staining (either nuclear or cytoplasmic), and staining intensity (Table S5, Figure S3). This evidence concerns the gene NFKB1 and gastric cancer.