Of seven cell lines selected for their experimental tractability (e.g. ease of transfection and convenient growth conditions), we found that both β-catenin and the TCF/LEF transcription factor TCF4 (also known as TCF7L2), major components of the Wnt signaling pathway, were expressed in GC cell lines predicted by the pathway activation analyses to have high Wnt/β-catenin activity (AGS, YCC3, Kato III, and NCI-N87), but not expressed in two out of three lines (SNU1 and SNU5) associated with inconsistent or low Wnt/β-catenin activation scores (Figure 4B). Here, TCF4 is linked to gastric cancer.