Overall, our results demonstrate that in vivo polarized Th2 CD4+ T cells specific for a tumor-associated antigen from PC patients are endowed with plasticity, and support further studies aimed at investigating the possible clinical application of the combination of IL-12 and IL-27 as a treatment for pancreatic cancer both as cytokines delivery and possibly for reprogramming tumor antigen specific T cells prior to adoptive immunotherapy. Here, IL27 is linked to neoplasm.