Further study on the mechanisms of action of BER induced apoptosis in the highly-metastatic human breast cancer cells showed that the expressions of anti-apoptotic protein Bcl-2 were down-regulated and the levels of pro-apoptotic protein Bax were up-regulated in both MDA-MB-231 and MDA-MB-435S cells after the cells were treated for 48 hours with BER at concentrations of 40 μM and 60 μM (Fig. 3A and 3B). The gene discussed is BCL2; the disease is breast cancer.