In fact, in the 4 related cell lines that arose from the same spontaneously arising cancer cell clone, mesenchymal gene expression (N-cadherin, vimentin) was greatest in the nonmetastatic cell line (67NR) and decreased with increased metastatic potential, while epithelial gene expression of E-cadherin, CK-18 and EGFR followed the opposite trend. This evidence concerns the gene VIM and cancer.