Our study aimed to elucidate serum levels of the most debated cytokines and chemokines including interleukin-1α (IL-1α), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), macrophage inflammatory protein (MIP-1α), monocyte chemoattractant protein (MCP-1), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) in subjects with DM and the healthy subjects by using the multiplex bead immunoassay. The gene discussed is CXCL8; the disease is diabetes mellitus.