To further confirm the inhibitory effect of FOXG1 on TGF-β signalling in ovarian cancer cells, we used the vector-based RNAi technique and successfully knockdown >75% endogenous FOXG1 in an FOXG1 overexpressing cell lines, SKOV3 and A2780cp (Figure 6A and B). Here, FOXG1 is linked to ovarian carcinoma.