Further, the level of changes in the transcripts exclusively induced in one or the other bacterium during infection of R. majalis was determined by quantitative real-time PCR (qRT-PCR) on following representative genes: cysK, def, dnaB, lysR, pchC, pckA, phoP, ptst1, sctL, selD, tolB and virB from P. temperata, and aceK, clpP, cobJ, dacC, dld, malF, res, rtxC, tilS, virH, xhlA, and xkst4 from X. koppenhoeferi. The gene discussed is KARS1; the disease is infection.