In particular, this work allowed the discovery of two novel biological processes involved in the cytotoxic effects of the N-BPs, DNA damage and microtubule assembly, and, thanks to the 'barcode' approach, these could be linked directly to the responsible genes, DBF4 and TBCB. In this case, a strong conservation between yeast and mammalian targets was seen, since their involvement was confirmed also in our human breast cancer cell line, MCF-7, used as a mammalian model. Here, DBF4 is linked to breast cancer.