Aberrant activating of Hh pathway leads transcription factor Gli1 into nucleus to bind the promoter region of target genes and promote gene transcriptions, which maintains the biologic behaviors of cancer cells.[29] Continuous activation of Shh-Gli1 signaling cascade has recently been implicated in the growth of a number of human malignancies.[30, 31] In our study, we validated reactivation of Shh-Gli1 signaling pathway in pancreatic cancer tissues and cultured cancer cells by testing the main components of Shh-Gli1 signaling pathway using qRT-PCR or IP-western. The gene discussed is GLI1; the disease is familial pancreatic carcinoma.