Thus, under the strict control, the negative amplification of GAPDH and positive amplification of Bcl-2 and IGFBP6 from DNA template recovered from immunoprecipitation compared with negative control provided evidence that transcription factor Gli1 bound to the promoters of Bcl-2 and IGFBP6 real time in the cultured pancreatic cancer cells [Figure 5B]. Here, GLI1 is linked to pancreatic neoplasm.