In present study, we examined the effect of Zen and α-Zel at different concentrations on T47D, estrogen receptor-positive, and MDA-MB-231, estrogen receptor-negative breast cancer cell lines as negative control cells, for in vitro model determination of these myco-estrogens and 17-β-Estradiol (E2) as positive control agent. Here, ESR1 is linked to breast cancer.