The importance of NFAT signalling in regulating COX-2 promoter activation and protein production was evaluated in BRAF-mutated melanoma cells using COX-2 luciferase reporter vectors that selectively lacked functional NFAT binding sites, calcineurin inhibitors and siRNA-mediated knockdown of NFAT2 and 4. The gene discussed is NFATC1; the disease is melanoma.