In order to confirm whether Prom1-expressing cells are essential for tumorigenesis, we established mouse glioma-initiating cell (GIC) lines by overexpressing both SV40 Large T antigen (SV40LT) and a constitutive-active form of H-Ras (HRasL61) in neural stem cells (NSCs), whose Prom1-expressing cells can be eliminated genetically. Here, PROM1 is linked to central nervous system cancer.