Furthermore, a significant correlation (Spearman r = 0.7832, P = 0.0026, n = 12) was observed between the IC50 values of 5-FU and pERK density values (Figure 4D), indicating that the resistance to 5-FU was significantly associated with basal pERK expression in these HCC cell lines. The gene discussed is EIF2AK3; the disease is hepatocellular carcinoma.