In this study, we have examined the contribution of monocyte-mediated TCR stimulation and cytokines including IL-2, IL-10 and TGFβ to the induction of CD4+CD25+Foxp3+ T cells using an in vitro co-culture system of trophozoite-stage malaria-infected red blood cells (iRBC) with PBMCs from healthy donors in the absence of any further stimulation or addition of exogenous cytokines. The gene discussed is CD4; the disease is malaria.