Logothetis et al. (1992) also reported that of their group of patients with locally advanced bladder cancer that had been treated by surgery and chemotherapy, the patients whose tumors expressed an altered Rb protein had significantly poorer tumor-free survival rates. These observations were supported by Cote et al. (1998), who examined the IHC expression of the p53 and Rb proteins from the bladder cancers of 185 patients who had undergone radical cystectomy. This evidence concerns the gene RB1 and urinary bladder cancer.