TIMP2 and systemic sclerosis: Matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinases-2 (TIMP-2) levels in patients with systemic sclerosis were determined by means of sandwich ELISA in a study that concluded that serum TIMP-2 level can be used as a marker of the extent of skin sclerosis and disease activity added to the fact that TIMP-2 and MMP-2’s balance may play an important role in those patients (Yazawa et al. 2000).