To examine the transcriptional levels of B4GALT1 and OSMR, we performed RT-PCR or Real-time RT-PCR analysis (Fig. 3) using cDNA prepared from CRC cell lines (HCT116, HT29, DLD-1, RKO, and SW480) and a non-tumorigenic cell line, HEK293. Here, B4GALT1 is linked to colorectal carcinoma.