RT-PCR results (Figure 4) confirm the upregulation of PTEN, FAS, as well as several pro-apoptotic transcriptional targets of FOXO3A in Tregs from T1D subjects (GADD45A, GADD45B, SESN1, PUMA and TNFRSF10B). This evidence concerns the gene FOXO3 and type 1 diabetes mellitus.