Amongst a subset of direct FOXP3 targets that exhibited consistent transcriptional behavior in hybridomas and in ex vivo T-cells [64], we found several negative targets of FOXP3 (EVI2B, GADD45B, PTPN22, TGIF, MYC, PHF6, POU2AF1 and GPR171) to be upregulated in Tregs from T1D subjects while positive FOXP3 targets (IL2RA, CD2 and CPEB2) were downregulated in Tregs from T1D subjects. This evidence concerns the gene PHF6 and type 1 diabetes mellitus.