VCAN and infection: Other systems that are important for infection and persistence in our model include carbon metabolism, with attenuating mutants found in a number of genes involved in glycolysis (pgm, ptsG, crr and tpiA), the TCA cycle (sucABC and sdhABC), mannose utilisation (mtlA, mtlD, rfbM and manA) and oxidative phosphorylation (the nuo locus, atpABFI, cyoAB and cydA[52]).