G6PD and sickle cell disease: From a biological perspective, the globin depleted PAX gene versus the PBMC difference expression profile provided a window into real time erythrocyte expression profiles in vascular diseases In clinical investigations on hematological diseases such as sickle cell anemia, thalassemia, G6PD, pyruvate kinase deficiency and in malaria where the early red blood cell progenitors represent major contributors to pathophysiology, insights into the transcription profile of these cells may contribute greatly to our understanding of mechanism of disease, prognosis, and responses to therapeutics.