The 100 genes representing hypothetical proteins, cDNA clones, unknown transcripts and genes such as hemochromatosis, hemoglobin epsilon 1 and transcribed locus similar to alpha hemoglobin that were detectable only in PAX samples were found to be masked or lost by globin reduction process as indicated by the inability to detect them in PAX-GR samples. The gene discussed is HBE1; the disease is hemochromatosis.