On the basis of previous work demonstrating that chronic acetaminophen (N-acetyl p-aminophenol, APAP) intake (30 mg/kg body weight/day) can be safely (e.g. in the absence of hepatotoxicity) used to prevent age-associated hyperglycemia [23], and other findings suggesting that elevated glucose levels can induce iNOS expression [19], [20] while reduce PTEN expression [14], we tested if this type of treatment regimen would also be effective in improving Akt function. Here, NOS2 is linked to Hyperglycemia.