To confirm the relevance of the expression of DNMT3A and the relationship between miR-143 and DNMT3A, we assessed the expressions of miR-143 and DNMT3A mRNA in the seven colon cancer cell lines and in an independent set of human CRC tumour tissues and their adjacent colonic tissues from 10 CRC Hong Kong patients. The gene discussed is DNMT3A; the disease is colorectal carcinoma.