Figure 4 shows that a partial shift in the FACS profile was observed for the colorectal cancer cell line LS174T, whereas a very marked increase of cell fluorescence was detected for the pVHL-defective human RCC cell line SK-RC-52, similar to what previously reported for the clinical-stage cG250 monoclonal antibody (Grabmaier et al, 2000). The gene discussed is VHL; the disease is renal cell carcinoma.