Nonetheless, the observed reduction in the frequency of loop formation between the D4Z4 array and the FRG1 promoter in FSHD myoblasts is too small to certainly infer its involvement in the misregulation of FRG1 gene expression, and thus further experiments are required to link macrosatellite contraction and gene expression. This evidence concerns the gene FRG1 and facioscapulohumeral muscular dystrophy.