In the present study, an alternative approach was chosen to define the priming effect of ANCA against PR3 (PR3-ANCA) from WG patients on inflammatory leukocyte functions: human PBMCs were preincubated with substimulatory concentrations of PR3-ANCA and possible augmented cell activation by various microbial PAMPs was examined. This evidence concerns the gene PRTN3 and granulomatosis with polyangiitis.