RT-PCR confirmed abundant expression of XIST mRNA in two female controls (N-020, N-031) and in two 46,XX virilized CAH-individuals (P-103, P-105) and showed an absence of expression in two 46,XY-controls (N-003, N-015) and two sex-reversed 46,XY DSD-females (P-019, P033). The gene discussed is XIST; the disease is disorder of sexual differentiation.