Transcripts specific for the four chains (ε, ζ, γ and δ) of CD3 complex, γTCR and δTCR constant regions and CD8 were amplified and quantified by real-time PCR in eosinophils purified to homogeneity (100%) and γδT lymphocyte subsets or Colo-205 colon carcinoma cells as positive and negative controls respectively. Here, CD8A is linked to colon carcinoma.