Our results also indicate that whereas the function of CD4/10.4 and CD8/10.4 cells may be partially overlapping in the early stages of infection (judged by the small differences in polyfunctionality, CD107a/b expression, and in vivo cytoxicity), at later time points these cells showed significant differences in these markers suggesting more disparate effector functions of the subsets in the late stages of infection. Here, CD4 is linked to infection.