As a next step towards development of gene therapy for arthritis, we constructed an rAAV5 vector containing the IKKβdn transgene (AAV5.IKKβdn) and demonstrated that local NF-κB blockade by IKKβdn using rAAV5 as a vector significantly reduced established arthritis in vivo in rats, resulting in a significant reduction of synovial inflammation. Here, NFKB1 is linked to Arthritis.