Meta-analysis which further combined the AKT1/rapamycin data with publicly available breast tumor data sets revealed that a subset of the AKT1-regulated genes, upregulated by AKT1 and decreased by rapamycin, were co-regulated in human tumors and were associated with ER negative status and poor prognosis, but the other subset downregulated by AKT1 was not [39], indicating that a combination of different methods produces a biologically more relevant gene set. This evidence concerns the gene AKT1 and breast neoplasm.