Moreover, cytoplasmic expression (71.2%) of Kaiso in the lung cancer samples from patients with lymph node metastases was significantly higher than that (54.2%) in samples from patients without lymph node metastases, suggesting that cytoplasmic Kaiso expression in primary cells was closely associated with tumor lymph node metastases (p = 0.003). This evidence concerns the gene ZBTB33 and neoplasm.