Significant enhancement for both the isotypes with a dominance of IgG2a were also observed in liposomal LD72 immunized groups with minimum production of the isotypes in liposomal LD91 group (p<0.001 compared to controls) These results further substantiate the magnitude of the production of both the cytokines, IFN-γ and IL-4, before infection is predictive of success of vaccination. The gene discussed is IFNG; the disease is infection.