Similarly, GFP-tagged mutant ataxin-3, containing an expanded polyglutamine tract (77Q) associated with MJD, predominately formed cytoplasmic aggregates in transfected ST14A cells over a similar time period whereas non-expanded ataxin-3 (24Q) localized diffusely (Fig. 1B). Here, ATXN3 is linked to Machado-Joseph disease.