When mice were infected with F. hepatica subsequent to infection with T. gondii, there was a slight, but statistically significant, reduction in the levels of T. gondii-specific IFNγ in co-infected mice compared with mice infected with T. gondii only (P<0.05; Fig. 5B) but the level of IFN-γ produced was still relatively high (at a mean of >30 ng/ml, it was actually higher than the values seen in samples taken from mice infected with F. hepatica prior to T. gondii, where the mean was just over 20 ng/ml). The gene discussed is IFNG; the disease is infection.