Tumor location had no effect on HMGB1 (ANOVA, p > 0.05) or CEA levels (ANOVA, p > 0.05) (Table 3), and in GC patients with metastatic lesions (M group), there was no difference in serum HMGB1 levels (ANOVA, p > 0.05) or CEA levels (ANOVA, p > 0.05) (Table 3) according to distant metastatic organs. The gene discussed is HMGB1; the disease is gastric cancer.