In this study, the IHC results of all the 4 individual markers (PR, ER, Vim and CEA), as well as their respective 3-marker (ER/Vim/CEA) or 4-marker (PR/ER/Vim/CEA) panels, showed significant differences in expression frequencies between the two types of adenocarcinomas (ECA vs. EMA). Here, ESR1 is linked to adenocarcinoma.