To further examine whether IGF-I activation of caspases 3/7, 8 and 9 increases apoptosis in colon cancer cells, HT-29, SW620 and HCT116 cells were treated with IGF-I (100 ng/ml) in SCM and SFM conditions respectively for 48 hours. The gene discussed is IGF1; the disease is malignant colon neoplasm.