Furthermore, a LOD score of less than 1 with all 23 markers involved did not support any linkage relation in this study, so we excluded the linkage of microphthalmia in this pedigree with the reported candidate genes (CHX10, PAX6, and MFRP) and loci (chromosomes 14q32, 11p, and 2q11-q14). This evidence concerns the gene MFRP and microphthalmia.