A second step of the test could be performed if positive IFN-γ/IP-10 results are obtained to measure three-marker combinations of EGF, sCD40L, MIP-1β, VEGF, IL-1α or TGF-α levels by ELISA or multiplex cytokine assays, to differentiate individuals with active TB from LTBI. This evidence concerns the gene CCL4 and tuberculosis.