We set a threshold of 0.2% IFNγ+, CD4+, CD45RO+ T cells (i.e. 20-fold above the assumed flow cytometric detection threshold of 0.01%) to define positive T-cell responses against latency-associated antigens in LTBI and TB patients for the most promising candidates (P<0.001) (Table 2). Here, CD4 is linked to tuberculosis.