To internally control experiments, mixed infections were performed with a 1:1 ratio of sporozoites of Lisp1ΔGreen and of WTRed, a WT ANKA derivative that expresses red fluorescent protein (RFP) from the eef1α promoter (Sturm et al., 2009) and the ratio of green versus red merozoites (G/R) released in the culture supernatant was calculated (see Table 1). Here, EEF1A1 is linked to infection.