The data, however, clearly showed that lymphatic subpopulation (CD3, CD4, CD16 and CD56) of patients undergoing the classic cholecystectomy contained, preoperatively, the higher quantities of perforin in their granules in comparison with patients selected for LC (Figures 5(a), 5(b), and 6). This evidence concerns the gene PRF1 and laryngotracheoesophageal cleft.