To assess these two profiles, we stimulated PBMC of HLA-A*0201 LTBI subjects and TB patients with the same individual peptides as those present in tetramers used in this study and determined the proportion of tetramer specific CD8 T-cells that produced IFN-γ and/or IL-2 by intracellular FACS analysis, after short-term stimulation with peptides. The gene discussed is IFNG; the disease is tuberculosis.