To understand the mechanisms and to provide proof of metastatic spread observed in the mice injected with INHα-positive cells, we stained LNCaP and PC3 INHα and EV orthotopic tumours for LYVE-1, and human mitochondrial antibody to determine LVD and the degree of invasion of tumour cells into lymphatic vessels (lymphatic invasion) in the tissues. This evidence concerns the gene LYVE1 and neoplasm.