Balb/c WT mice and MUC1 transgenic Balb/c mice that had been vaccinated with Theratope or controls, as described above, were tumour challenged with mammary carcinoma cell line expressing MUC1 and ST6GalNAc 1 (E3-STn) or E3 that express human MUC1 without STn. Here, MUC1 is linked to breast carcinoma.